pWY025 pEF1a_LplA-HA-GFP

LOCUS       pWY025 pEF1a_LplA-HA-GFP               9600 bp DNA     circular SYN 13-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(68..928)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     691..710
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(929..1033)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1101..1119
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1157..1179)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1181..1205
                     /label=Seq-primer F
     primer_bind     1256..1278
                     /label=Primer 1
     primer_bind     1297..1336
                     /label=Fragment.FOR
     misc_feature    1317..2780
                     /standard_name="L1: oriT traJ + CEN ARS HIS (concatenated 
                     sequence 2)"
                     /label=L1-pICH47732
                     /note="B/3"
     CDS             complement(1394..1765)
                     /codon_start=1
                     /gene="traJ"
                     /standard_name="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            1798..1907
                     /standard_name="incP origin of transfer oriT"
                     /label=incP origin of transfer oriT
                     /note="incP origin of transfer"
     misc_feature    2043..2046
                     /standard_name="Ligation"
                     /label=Ligation
                     /note="Geneious type: ligation"
     regulatory      2090..2593
                     /standard_name="CEN/ARS"
                     /label=CEN/ARS
     misc_feature    2090..2593
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     complement(2184..2215)
                     /label=Primer 3
     misc_feature    2303
                     /standard_name="A"
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     promoter        2594..2780
                     /gene="S. cerevisiae HIS3"
                     /standard_name="HIS3 promoter"
                     /label=HIS3 promoter
     primer_bind     2674..2700
                     /label=S11F
     primer_bind     2761..2802
                     /label=C18F
     promoter        2781..3380
                     /label=pro-EF1a
     misc_feature    3049
                     /label=T
     primer_bind     3251..3272
                     /label=S13F
     primer_bind     3309..3336
                     /label=W3a
     primer_bind     complement(3340..3389)
                     /label=Fragment 1.REV
     primer_bind     3362..3383
                     /label=LplA-F2
     primer_bind     3372..3402
                     /label=C19F
     CDS             3381..3632
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             3939..4832
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     primer_bind     3939..3963
                     /label=W3b
     misc_feature    4243
                     /label=T to G mutation
     primer_bind     4612..4637
                     /label=W3c
     primer_bind     complement(4807..4842)
                     /label=C19R
     primer_bind     4824..4872
                     /label=C20F
     misc_feature    4833..5073
                     /label=fcpA terminator
     primer_bind     complement(5048..5086)
                     /label=C20R
     primer_bind     5066..5091
                     /label=seq-p26-F2
     primer_bind     5069..5100
                     /label=C23F
     misc_feature    5084..5492
                     /label=Feature 25
     primer_bind     5101..5114
                     /label=seq-p26-F1
     misc_feature    5157..5305
                     /label=tetra-LexA operator
     primer_bind     5299..5327
                     /label=S18F
     promoter        5388..5434
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     5447..5466
                     /label=35S-F
     primer_bind     complement(5468..5492)
                     /label=35S-lplA-R
     primer_bind     complement(5471..5492)
                     /label=C23R
     primer_bind     5472..5513
                     /label=LplA-35s-F
     CDS             5493..6509
                     /codon_start=1
                     /label=LPLA
                     /translation="MGSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTISTS
                     IVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLSRL
                     ANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEAEII
                     SPNKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHITRAQ
                     VFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMAGAVR"
     primer_bind     5493..5522
                     /label=NcoI-F
     primer_bind     5493..5513
                     /label=LplA-F2
     misc_feature    5493..5496
                     /label=HSP70-signal peptide
     primer_bind     5496..5516
                     /label=LplA-F1
     primer_bind     complement(6210..6236)
                     /label=LplA-N-R
     misc_feature    6237..6509
                     /label=C domain
     primer_bind     6237..6260
                     /label=lplA-C-F
     primer_bind     complement(6490..6529)
                     /label=LplA-R1
     primer_bind     complement(6491..6509)
                     /label=LplA-his R
     primer_bind     complement(6492..6511)
                     /label=XhoI-R
     primer_bind     complement(6509..6535)
                     /label=Primer 2
     primer_bind     complement(6509..6535)
                     /label=Fragment.REV (2)
     CDS             6510..6536
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6510..6529
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVP"
     primer_bind     6510..6528
                     /label=vector-lpla-r1
     primer_bind     6510..6528
                     /label=Vector-lplA-R
     CDS             6540..6566
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6569..6613)
                     /label=Primer 2
     primer_bind     complement(6569..6596)
                     /label=Fragment.REV (2)
     CDS             6570..6596
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6586..6605)
                     /label=V-PEF1A-R
     misc_feature    6597..7313
                     /label=eGFP
     primer_bind     6597..6614
                     /label=Fragment.FOR
     primer_bind     complement(6634..6653)
                     /label=GEP-IN-R
     misc_feature    7212..7214
                     /label=from K(AAG) to A(GCC)
     primer_bind     complement(7290..7328)
                     /label=C24R
     primer_bind     complement(7311..7329)
                     /label=C23R
     primer_bind     complement(7328..7344)
                     /label=Fragment.REV (2)
     primer_bind     complement(7413..7439)
                     /label=seq-p26-R1
     primer_bind     complement(7413..7439)
                     /label=Seq-Primer R
     misc_feature    7448..7688
                     /label=fcpA terminator
     primer_bind     7448..7487
                     /label=C20F
     primer_bind     complement(7663..7689)
                     /label=C20R
     misc_feature    7689..7927
                     /label=pfcpB
     primer_bind     7689..7710
                     /label=Primer 2
     misc_feature    7946..8368
                     /label=bsr
     primer_bind     8382..8422
                     /label=C20F
     misc_feature    8383..8623
                     /label=fcpA terminator
     primer_bind     complement(8598..8623)
                     /label=C20R
     primer_bind     complement(8632..8651)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8633..8651)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8738..8755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8909..9497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8989..9008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
       61 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
      121 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
      181 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
      241 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
      301 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
      361 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
      421 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
      481 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
      541 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
      601 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
      661 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
      721 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
      781 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
      841 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
      901 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
      961 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     1021 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     1081 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     1141 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     1201 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     1261 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatggcct
     1321 cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta
     1381 gcggctaaaa aagtcatggc tctgccctcg ggcggaccac gcccatcatg accttgccaa
     1441 gctcgtcctg cttctcttcg atcttcgcca gcagggcgag gatcgtggca tcaccgaacc
     1501 gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag gccgcccagg cggcccaggt
     1561 cgccattgat gcgggccagc tcgcggacgt gctcatagtc cacgacgccc gtgattttgt
     1621 agccctggcc gacggccagc aggtaggccg acaggctcat gccggccgcc gccgcctttt
     1681 cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg ctgcccttcc
     1741 tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg gcggtagccg
     1801 gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga
     1861 agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt
     1921 ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa
     1981 aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat gcagcggaag
     2041 atttgtatgt gtccagcaaa gtagtagctg ctccagttct cccgcgagca tcacgtgcta
     2101 taaaaataat tataatttaa attttttaat ataaatatat aaattaaaaa tagaaagtaa
     2161 aaaaagaaat taaagaaaaa atagtttttg ttttccgaag atgtaaaaga ctctaggggg
     2221 atcgccaaca aatactacct tttaccttgc tcttcctgct ctcaggtatt aatgccgaat
     2281 tgtttcatct tgtctgtgta gatgaccaca cacgaaaatc ctgtgatttt acattttact
     2341 tatcgttaat cgaatgtata tctatttaat ctgcttttct tgtctaataa atatatatgt
     2401 aaagtacgct ttttgttgaa attttttaaa cctttgttta tttttttttc ttcattccgt
     2461 aactcttcta ccttctttat ttactttcta aaatccaaat acaaaacata aaaataaata
     2521 aacacagagt aaattcccaa attattccat cattaaaaga tacgaggcgc gtgtaagtta
     2581 caggcaagcg atcctagtac actctatatt tttttatgcc tcggtaatga ttttcatttt
     2641 ttttttccac ctagcggatg actctttttt tttcttagcg attggcatta tcacataatg
     2701 aattatacat tatataaagt aatgtgattt cttcgaagaa tatactaaaa aatgagcagg
     2761 caagataaac gaaggcaaag cgaaacgaat agaagctccc cgaggtcggg tgttgtttgg
     2821 gaggttcatg gtggtttcgg tgtcgcttgc tcgctcgctc gttcgcagtg acagacagtt
     2881 cgtgagacac gagaaccgtt ggcgtccgag ttcgggtgcc gcatttcgtc gtctccacga
     2941 ttcaattctt gcccatcaga cgagtcccga attccgtgac tctggatgcg atttactgtc
     3001 caaacgaaac ccaacgattc cgtacgttgt tttctatttt acagtgagac ttcgatacca
     3061 ccgtacaacc atcgttcgtg taccgtctgg tagtcccacg tgtcgacaac gtgtggctct
     3121 ggaccgatga gttgtttgcc gttcggaaac gagcagtacc aaggaattca cagaaacaca
     3181 gcccatgtaa cacaacgacc gcgaatcgtt tcggtgctct cgcttcgcgt acgggcgggc
     3241 ggtccccccc aagcagcgag aggagtccgc agcgtcatag ttgcattccg gggcccactc
     3301 gcgttgttca ctctctcgtc tagtagagaa acttccatcg gatcgtatca taatattgta
     3361 tcgtataata tcacgtaatc atgaaagcgt taacggccag gcaacaagag gtgtttgatc
     3421 tcatccgtga tcacatcagc cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc
     3481 gtttggggtt ccgttcccca aacgcggctg aagaacatct gaaggcgctg gcacgcaaag
     3541 gcgttattga aattgtttcc ggcgcatcac gcgggattcg tctgttgcag gaagaggaag
     3601 aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc gtcgagcgcc cccccgaccg
     3661 atgtcagcct gggggacgag ctccacttag acggcgagga cgtggcgatg gcgcatgccg
     3721 acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg ggtccgggat
     3781 ttacccccca cgactccgcc ccctacggcg ctctggatat ggccgacttc gagtttgagc
     3841 agatgtttac cgatgccctt ggaattgacg agtacggtgg ggatccgtct gctggagaca
     3901 tgagagctgc caacctttgg ccaagcccgc tcatgatcaa acgctctaag aagaacagcc
     3961 tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct gagcccccca
     4021 tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg atgggcttac
     4081 tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag agggtgccag
     4141 gctttgtgga tttgaccctc catgatcagg tccaccttct agaatgtgcc tggctagaga
     4201 tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agggaagcta ctgtttgctc
     4261 ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg gagatcttcg
     4321 acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga gaggagtttg
     4381 tgtgcctcaa atctattatt ttgcttaatt ctggagtgta cacatttctg tccagcaccc
     4441 tgaagtctct ggaagagaag gaccatatcc accgagtcct ggacaagatc acagacactt
     4501 tgatccacct gatggccaag gcaggcctga ccctgcagca gcagcaccag cggctggccc
     4561 agctcctcct catcctctcc cacatcaggc acatgagtaa caaaggcatg gagcatctgt
     4621 acagcatgaa gtgcaagaac gtggtgcccc tctatgacct gctgctggag atgctggacg
     4681 cccaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggag acggaccaaa
     4741 gccacttggc cactgcgggc tctacttcat cgcattcctt gcaaaagtat tacatcacgg
     4801 gggaggcaga gggtttccct gccacagtct gaccttcctt aaaaatttaa ttttcattag
     4861 ttgcagtcac tccgctttgg tttcacagtc aggaataaca ctagctcgtc ttcaccatgg
     4921 atgccaatct cgcctattca tggtgtataa aagttcaaca tccaaagcta gaacttttgg
     4981 aaagagaaag aatatccgaa tagggcacgg cgtgccgtat tgttggagtg gactagcaga
     5041 aagtgaggaa ggcacaggat gagttttctc gagagctcgg tactcgacag cttgcatgcc
     5101 gcttgggctg caggtcgagg ctaaaaaact aatcgcatta tcatcccctc gacgtactgt
     5161 acatataacc actggtttta tatacagcag tactgtacat ataaccactg gttttatata
     5221 cagcagtcga cgtactgtac atataaccac tggttttata tacagcagta ctgtacatat
     5281 aaccactggt tttatataca gcagtcgagg taagattaga tatggatatg tatatggata
     5341 tgtatatggt ggtaatgcca tgtaatatgc tcgactctag gatcttcgca agacccttcc
     5401 tctatataag gaagttcatt tcatttggag aggacacgct gaagctagtc gactctagcc
     5461 tcgaggcgcg ccaggcctac gcgtttaatt aaatgggctc cacattacgc ctgctcatct
     5521 ctgactctta cgacccgtgg tttaacctgg cggtggaaga gtgtattttt cgccaaatgc
     5581 ccgccacgca gcgcgttctg tttctctggc gcaatgccga cacggtagta attggtcgcg
     5641 cgcagaaccc gtggaaagag tgtaataccc ggcggatgga agaagataac gtccgcctgg
     5701 cgcggcgcag tagcggtggc ggcgcggtgt tccacgatct cggcaatacc tgctttacct
     5761 ttatggctgg caagccggag tacgataaaa ctatctccac gtcgattgtg ctcaatgcgc
     5821 tgaacgcgct cggcgtcagc gccgaagcgt ccggacgtaa cgatctggtg gtgaaaaccg
     5881 tcgaaggcga ccgcaaagtc tcaggctcgg cctatcgcga aaccaaagat cgcggcttcc
     5941 accacggcac cttgctactc aatgccgacc tcagccgcct ggcaaactat ctcaatccgg
     6001 ataaaaagaa actggcggcg aaaggcatta cgtcggtacg ttcccgcgtg accaacctca
     6061 ccgagctgtt gccggggatc acccatgagc aggtttgcga ggccataacc gaggcctttt
     6121 tcgcccatta tggcgagcgc gtggaagcgg aaatcatctc cccgaacaaa acgccagact
     6181 tgccaaactt cgccgaaacc tttgcccgcc agagtagctg ggaatggaac ttcggtcagg
     6241 ctccggcatt ctcgcatctg ctggatgaac gctttacctg gggcggcgtg gaactgcatt
     6301 tcgacgttga aaaaggccat atcacccgcg cccaggtgtt taccgacagc ctcaaccccg
     6361 cgccgctgga agccctcgcc ggacgactgc aaggctgcct gtaccgcgca gatatgctgc
     6421 aacaggagtg cgaagcgctg ttggttgact tcccggaaca ggaaaaagag ctacgggagt
     6481 tatcggcatg gatggcgggg gctgtaaggt acccgtatga tgttccggat tacgctggct
     6541 atccctacga cgtgcccgac tatgccgggt acccctatga cgtcccagac tacgcagtga
     6601 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     6661 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     6721 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     6781 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     6841 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     6901 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     6961 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     7021 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     7081 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     7141 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     7201 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     7261 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tagtgtaccc
     7321 ggggatcctc tagagtcgac ctgcaggcat gcaagcttca gaagcgtgct atcgaactca
     7381 accagggacg tgcggcacaa atgggcatcc ttgctctcat ggtgcacgaa cagttgggag
     7441 tctctatcct tccttaaaaa tttaattttc attagttgca gtcactccgc tttggtttca
     7501 cagtcaggaa taacactagc tcgtcttcac catggatgcc aatctcgcct attcatggtg
     7561 tataaaagtt caacatccaa agctagaact tttggaaaga gaaagaatat ccgaataggg
     7621 cacggcgtgc cgtattgttg gagtggacta gcagaaagtg aggaaggcac aggatgagtt
     7681 ttctcgagac ataccttcag cgtcgtcttc actgtcacag tcaactgaca gtaatcgttg
     7741 atccggagag attcaaaatt caatctgttt ggacctggat aagacacaag agcgacatcc
     7801 tgacatgaac gccgtaaaca gcaaatcctg gttgaacacg tatccttttg ggggcctccc
     7861 gctacgacgc tcgctccagc tggggcttcc ttactataca cagcgcgcat atttcacggt
     7921 tgccagatgt caagacaatt ggtttatgaa aacgtttaat atctcgcaac aagaccttga
     7981 acttgtcgaa gtcgcaacgg aaaaaattac gatgctttac gaagacaata aacatcatgt
     8041 cggagcagca attcgaacga aaacgggaga aattatttcg gcagtccata ttgaagcata
     8101 cattggacga gtcacggtct gcgcagaagc aattgcaatt ggatcggcag tctcgaatgg
     8161 acaaaaagac tttgacacga ttgtcgcagt ccgacatcca tactcggacg aagtcgatcg
     8221 atcgattcga gtcgtctcgc catgcggaat gtgccgagaa cttatttcgg actacgcacc
     8281 agactgcttt gtccttattg aaatgaatgg aaaacttgtc aaaacgacga ttgaagaact
     8341 tattccactt aaatacacgc gaaattgaaa accaggactg aaccttcctt aaaaatttaa
     8401 ttttcattag ttgcagtcac tccgctttgg tttcacagtc aggaataaca ctagctcgtc
     8461 ttcaccatgg atgccaatct cgcctattca tggtgtataa aagttcaaca tccaaagcta
     8521 gaacttttgg aaagagaaag aatatccgaa tagggcacgg cgtgccgtat tgttggagtg
     8581 gactagcaga aagtgaggaa ggcacaggat gagttttctc gaggccggtc tccctatagt
     8641 gagtcgtatt aatttcgata agccaggtta acctgcatta atgaatcggc caacgcgcgg
     8701 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8761 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8821 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8881 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8941 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9001 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9061 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9121 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9181 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9241 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9301 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9361 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9421 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9481 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9541 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
//